By J. Greenstein, et al.,

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Extra resources for Advances in Cancer Research [Vol II]

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34 PETER ALEXANDER Changes in biological activity such as immunological reactions and enzyme inhibition are beyond the scope of this review. From general considerations one would expect that a t the physiological pH the nucleophilic alkylating agents will react with carboxyl groups, which are largely dissociated, as well as with terminal amino and the imidazole groups of histidine, which are appreciably un-ionized. No reaction would be expected with the €-amino groups of lysine, the guanidinium group of arginine, the phenolic group of tyrosine and the sulfhydryl group of cysteine, all of which exist almost entirely in their unreactive forms a t pH 7 (see p.

These authors showed that for asymmetric particles which are randomly coiled and easily deformed the viscosity is independent of shear, whereas for particles which resist deformation sheardependence is to be expected. However, even with rigid rodlike particles, the viscosity would not decrease as a result of orientation to less than half on going from zero to high rates of shear. Variations of this order have been observed for polymers the molecular weight of which does not exceed a few hundred thousand and show clearly that orientation of the molecules is not sufficient to explain the magnitude of the anomaly found with molecules such as DNA for which a different mechanism must be operative.

At this concentration the reaction with PMA is exclusively intramolecular. Although the effect of concentration on the reaction of deoxyribonucleic acid with mustard gas has not been determined systematically, a change-over from intra- to intermolecular reaction appears to occur as the concentration is increased. Elmore et al. (1948) noted a considerable increase in molecular weight with a 35 % solution of nucleic acid, whereas with solutions containing less than 1 % the reaction with mustard gas and HN2 is now thought to be intramolecular (see p.

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